other:Article Title: MEDAG functions as an A-kinase-anchoring protein in adipocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER InstantBlue Coomassie Protein Stain Abcam Cat# ab119211 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000111 High-Capacity cDNA RT kit Applied Biosystems N/A Glycerol reagent Sigma-Aldrich Cat# F6428 XFE96 FluxPak Agilent Cat# 102416-100 PKA kinase activity kit Enzo Life Sciences Cat# ADI-EKS-390A cAMP ELISA kit Enzo Life Sciences Cat# ADI-900-067A SuperSepTM Phos-tagTM Precast Gels Fujifilm Cat# 195-17991 ZebaTM Spin Desalting Columns, 7K MWCO Thermo Fischer Cat# 89883 Deposited data Unprocessed and uncompressed imaging data Mendeley Data https://doi.org/10.17632/vctw9w8bhs.1 Human BAT and subcutaneous WAT (human cohort 1) European Nucleotide Archive accession code: PRJEB20634 Human BAT (human cohort 2) Gene Expression Omnibus accession code: GSE220158 Experimental models: Cell lines HEK293-LTV Cell Biolabs Cat# LTV-100 HEK293 ATCC Cat# CRL-1573 immortalized brown adipocytes Kahn et al. 55 N/A 3T3-LA preadipocytes Thiele et al. 56 N/A Experimental models: Organisms/strains Adipoq-CreERT2: C57BL/6-Tg (Adipoq-icre/ERT2)1Soff/J Wolfrum et al. 57 N/A Medag fl/fl : C57BL/6N-Atm1Brd Medagtm2a(KOMP)Wtsi/Mmucd MMRRC Cat# 048575-UCD Adipoq-CreERT2 X Medag fl/fl Christian Wolfrum This paper Oligonucleotides qPCR primers Microsynth Listed in Table S2 siRNAs Microsynth Listed in Table S1 Primers for molecular cloning Microsynth Listed in Table S3 Recombinant DNA plenti-PKA-RIIβ-V5 Christian Wolfrum This paper pLenti-PKA-Cα-HA Christian Wolfrum This paper plenti-MEDAG wildtype -FLAG Christian Wolfrum This paper plenti-MEDAG 251A -FLAG Christian Wolfrum This paper plenti-MEDAG 251D -FLAG Christian Wolfrum This paper pMD2.G Turelli et al. 58 Didier Trono Addgene plasmid Cat# 12259 psPAX2 Turelli et al. 58 Didier Trono Addgene plasmid Cat# 12260 pLenti-CMV-MCS-BSD vector Witwicka et al. 59 Paul Odgren Addgene plasmid Cat# 73582 Software and algorithms ImageJ NIH, USA https://imagej.net/ij/ (Continued on next page) e3 Molecular Cell 86, 937–953.e1–e9, March 5, 2026
Article Title: A single-cell CRISPR screen defines a gene regulatory network governing human pluripotency in primed and naive cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hpES10 Amit et al.63 N/A HEK293T Laboratory of Robert Weinberg RRID: CVCL_0063 primed Cas9-EGFP-hPSCs This study N/A primed ZIM3-dCas9-mCherry-hPSCs Sherman et al.64 N/A Oligonucleotides sgRNA targeting ZNF281 (CRISPRi protospacer) hCRISPRi-v2 5′-GTATTAACTGGAGGCGACGG-3′ sgRNA targeting ETV4 (CRISPRi protospacer) hCRISPRi-v2 5′-GCCTCAGGTGAGGCTGCGGG-3′ sgRNA targeting Gal4 promoter (CRISPRi negative control protospacer) hCRISPRi-v2 5′-GAACGACTAGTTAGGCGTGT-3′ sgRNA targeting SMARCC1 (CRISPR KO; perturb-seq gRNA_10) This study See Table S2 sgRNA targeting TGIF1 (CRISPR KO; perturb-seq gRNA_3) This study See Table S2 sgRNA targeting ZNF281 (CRISPR KO; perturb-seq gRNA_7) This study See Table S2 Perturb-seq gRNA library composition This study See Table S2 qPCR primers: POU5F1_F This study 5′-GTGGAGGAAGCTGACAACAA-3′ qPCR primers: POU5F1_R This study 5′-TCTCCAGGTTGCCTCTCACT-3′ qPCR primers: NANOG_F This study 5′-CATGAGTGTGGATCCAGCTTG-3′ qPCR primers: NANOG_R This study 5′-CCTGAATAAGCAGATCCATGG-3′ qPCR primers: PRDM14_F This study 5′-ACCGGCCTCACAAGTGTTCT-3′ qPCR primers: PRDM14_R This study 5′-GAGTGCTGCCTGATGTGTGT-3′ qPCR primers: ETV4_F This study 5′-CACCTTCAGCAGCAAATCGC-3′ qPCR primers: ETV4_R This study 5′-ATCTTCAGAGTCGAGGGGCG-3′ qPCR primers: ZNF281_F This study 5′-CGCAATGCGTGTTATCCTCC-3′ qPCR primers: ZNF281_R This study 5′-CCGCGGGTTTCTCCTGTTTG-3′ qPCR primers: GAPDH_F This study 5′-AGCCACATCGCTCAGACACC-3′ qPCR primers: GAPDH_R This study 5′-GTACTCAGCGCCAGCATCG-3′ Recombinant DNA lentiCas9-EGFP A gift from Phil Sharp & Feng Zhang Addgene plasmid #63592 pCMV-VSV-G A gift from Bob Weinberg Addgene plasmid #8454 psPAX2 A gift from Didier Trono Addgene plasmid #12260 lentiCRISPR v2 A gift from Feng Zhang Addgene plasmid #52961 lentiGuide-Puro A gift from Feng Zhang Addgene plasmid #52963 pSpCas9(BB)-2A-GFP (PX458) A gift from Feng Zhang Addgene plasmid #48138 Software and algorithms Cell Ranger 10x Genomics version 6.1.2 Seurat Hao et al.65 version 5.1.0 Mixscape Papalexi et al.23 N/A limma Ritchie et al.66 N/A edgeR Robinson et al.67 N/A SCEPTRE Barry et al.68 N/A clusterProfiler Wu et al.69 N/A Bowtie 2 Langmead and Salzberg70 N/A MACS2 – v2.2.7.1 Picard Tools Broad institute https://broadinstitute.github.io/picard/ ChIPseeker Bioconductor v1.40.0 (Continued on next page) 20 Cell Reports 45, 117124, April 28, 2026
Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA myc(6) Ccpg1; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) Ccpg1 NTD; Mouse Ccpg1 (1-239) This paper N/A pcDNA myc(6) Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pcDNA myc(6) Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pcDNA myc(6) Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pcDNA myc(6) Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pcDNA myc(6) Fam134b; Mouse Fam134b This paper N/A pcDNA myc(6) Fam134c; Mouse Fam134c This paper N/A pcDNA myc(6) Sec62; Mouse Sec62 This paper N/A pcDNA myc(6) SQSTM1; Human SQSTM1 This paper N/A pcDNA myc(6) Calcoco1; Mouse Calcoco1 This paper N/A psPAX2 A gift from Didier Trono Addgene Plasmid #12260 pMD2.G A gift from Didier Trono Addgene Plasmid #12259 Software and algorithms ImageJ NIH, Scheneider et al. 79 https://imagej.net/ij/ Mito-QC counter (ImageJ) Montava-Garriga et al. 80 https://github.com/graemeball/mQC_ counter Imaris Oxford instruments https://imaris.oxinst.com/ QuPath Bankhead et al. 81 https://qupath.github.io/ NDP.view2 software Hamamatsu Photonics https://www.hamamatsu.com/jp/en/ product/life-science-and-medicalsystems/digital-slide-scanner/U1238801.html Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Graphpad GraphPad Software https://www.graphpad.com Fluoview FV31S-SW Olympus https://www.olympus-lifescience.com/en/ laser-scanning/fv3000/ LAS X software Leica Microsystems UK https://www.leica-microsystems.com/ products/microscope-software/p/leicalas-x-ls/ Nikon NIS-Elements Advanced Research Nikon https://www.microscope.healthcare.nikon. com/en_EU/products/software/niselements/nis-elements-advanced-research Zeiss Zen Slidescan Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html mRNA-Seq pipeline (nf-core/ rnaseq v3.12.0) Ewels et al. 82 https://doi.org/10.5281/zenodo.1400710 Nextflow Di Tommaso et al. 83 https://nextflow.io/ Trim Galore Braham Bioinformatics https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ STAR Dobin et al. 84 http://code.google.com/p/rna-star/ Salmon Patro et al. 85 https://github.com/COMBINE-lab/salmon.
Recombinant:Article Title: Nuclear-lamin-guided plastic positioning and folding of the human genome.
Article Snippet: .. This paper N/A Recombinant DNA Cas9 plasmid Addgene Addgene plasmid #87109 pCAG-mCherry-gRNA vector Addgene Addgene plasmid #87110 psPAX2 A gift from Didier Trono Addgene plasmid #12260 pMD2.G A gift from Didier Trono Addgene plasmid #12259 pLgw V5-EcoDam A gift from Bas van Steesel N/A pLgw EcoDam-V5-EMD A gift from Bas van Steesel N/A Software and algorithms Image Lab (version: 4.0) Bio-Rad http://www.bio-rad.com/zh-cn/product/ imagelabsoftware?ID=KRE6P5E8Z ImageJ (version: 1.8.0) NIH https://imagej.net/ij/ FlowJo (version: 10.0.7) FlowJo, LLC https://www.flowjo.com/ Leica LAS AF Lite (version: 2.6.0) Leica N/A GraphPad Prism 9 GraphPad Software Inc. https://www.graphpad.com/ ZEN 3.1 ZEISS https://www.micro-shop.zeiss.com/en/us/ system/zen+software/software+zen/zen++basic+software/410135-1002-310 Phenochart TM 1.0 Phenochart https://www.akoyabio.com/support/ software/phenochart-whole-slide-viewer/ TrimGalore (version 0.6.7) Babraham Bioinformatics https://github.com/FelixKrueger/ TrimGalore R (version 4.0.5 and 3.5) N/A https://www.r-project.org/ pheatmap (version 1.0.12) N/A https://cran.r-project.org/web/packages/ pheatmap/ ggplot2 (version 3.3.5) N/A https://cran.r-project.org/web/packages/ ggplot2/ Metascape (version 3.5) Zhou et al. 62 http://metascape.org/ Fastp (version 0.19.10) Chen et al. 63 https://github.com/OpenGene/fastp featureCounts (version 1.6.4 and 2.0.1) Liao et al. 64 http://subread.sourceforge.net/ featureCounts.html STAR (version 2.7.1a) Dobin et al. 65 https://github.com/alexdobin/STAR DESeq2 (version 1.30.1) Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html ggpubr (version 0.4.0) N/A https://cran.microsoft.com/snapshot/ 2021-09-26/web/packages/ggpubr/index. html deepTools (version 3.5.1) Ramirez et al. 67 https://github.com/deeptools/deepTools Bowtie2 (version 2.2.9) Langmead et al. 68 https://github.com/BenLangmead/bowtie2 MACS2 (version 2.2.6) Zhang et al. 69 https://github.com/macs3-project/MACS/ wiki/Install-macs2 SICER (version 0.1.1) Zang et al. 70 https://github.com/zanglab/SICER2 DiffBind (version 2.10.0) Ross-Innes et al. 71 https://bioconductor.org/packages/ release/bioc/html/DiffBind.html (Continued on next page) Cell Reports 44, 116529, November 25, 2025 23 ..
Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides See Table S3 for the sequence information of qPCR primers, shRNAs, siRNAs and sgRNAs This study N/A Recombinant DNA pMSCV-F-del-Casp9-IRES-GFP Straathof et al. 98 Addgene plasmid# 15567 rtTA-N144 Richner et al. 99 Addgene plasmid# 66810 pXG237 Guo et al. 40 Addgene plasmid# 141281 pRK5-HA-Ubiquitin-WT Lim et al. 100 Addgene plasmid# 17608 pMDLg/pRRE Dull et al. 101 Addgene plasmid# 12251 pRSV-Rev Dull et al. 101 Addgene plasmid# 12253 psPAX2 A gift from Didier Trono Addgene plasmid# 12260 pMD2.G A gift from Didier Trono Addgene plasmid# 12259 pVSV-G Dull et al. 101 Addgene plasmid# 138479 pLKO.1 Moffat et al. 102 Addgene plasmid# 10878 pHAGE-CMV-MCS-PGK-Puro Clontech Laboratories N/A pGEX-6P-1-GST Cytiva Cat# 28954648 pET-28a(+)-HIS MERCK Cat# 69864 pCMV-Myc Takara Bio Cat# 635689 Myc-VCP and mutants This study N/A uORFs-iCasp9-HA This study N/A pHAGE-PGAM5-3xFlag and mutants This study N/A GST-PGAM5 and mutants This study N/A His-OMA1 This study N/A His-PO1 This study N/A HA-OMA1 and mutants This study N/A pHAGE-DELE1-3xFlag This study N/A Software and algorithms Maestro Schrö dinger https://www.schrodinger.com/platform/ products/maestro/ GraphPad Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ GSEA Broad Institute http://software.broadinstitute.org/gsea/ login.jsp ImageJ ImageJ https://imagej.nih.gov/ij/ ZEN 2.3 (blue edition) Carl Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html R version 4.4.0 The R Foundation https://www.r-project.org/ Neuron 114, 1–19.e1–e14, June 17, 2026 e6 ..
Plasmid Preparation:Article Title: Nuclear-lamin-guided plastic positioning and folding of the human genome.
Article Snippet: .. This paper N/A Recombinant DNA Cas9 plasmid Addgene Addgene plasmid #87109 pCAG-mCherry-gRNA vector Addgene Addgene plasmid #87110 psPAX2 A gift from Didier Trono Addgene plasmid #12260 pMD2.G A gift from Didier Trono Addgene plasmid #12259 pLgw V5-EcoDam A gift from Bas van Steesel N/A pLgw EcoDam-V5-EMD A gift from Bas van Steesel N/A Software and algorithms Image Lab (version: 4.0) Bio-Rad http://www.bio-rad.com/zh-cn/product/ imagelabsoftware?ID=KRE6P5E8Z ImageJ (version: 1.8.0) NIH https://imagej.net/ij/ FlowJo (version: 10.0.7) FlowJo, LLC https://www.flowjo.com/ Leica LAS AF Lite (version: 2.6.0) Leica N/A GraphPad Prism 9 GraphPad Software Inc. https://www.graphpad.com/ ZEN 3.1 ZEISS https://www.micro-shop.zeiss.com/en/us/ system/zen+software/software+zen/zen++basic+software/410135-1002-310 Phenochart TM 1.0 Phenochart https://www.akoyabio.com/support/ software/phenochart-whole-slide-viewer/ TrimGalore (version 0.6.7) Babraham Bioinformatics https://github.com/FelixKrueger/ TrimGalore R (version 4.0.5 and 3.5) N/A https://www.r-project.org/ pheatmap (version 1.0.12) N/A https://cran.r-project.org/web/packages/ pheatmap/ ggplot2 (version 3.3.5) N/A https://cran.r-project.org/web/packages/ ggplot2/ Metascape (version 3.5) Zhou et al. 62 http://metascape.org/ Fastp (version 0.19.10) Chen et al. 63 https://github.com/OpenGene/fastp featureCounts (version 1.6.4 and 2.0.1) Liao et al. 64 http://subread.sourceforge.net/ featureCounts.html STAR (version 2.7.1a) Dobin et al. 65 https://github.com/alexdobin/STAR DESeq2 (version 1.30.1) Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html ggpubr (version 0.4.0) N/A https://cran.microsoft.com/snapshot/ 2021-09-26/web/packages/ggpubr/index. html deepTools (version 3.5.1) Ramirez et al. 67 https://github.com/deeptools/deepTools Bowtie2 (version 2.2.9) Langmead et al. 68 https://github.com/BenLangmead/bowtie2 MACS2 (version 2.2.6) Zhang et al. 69 https://github.com/macs3-project/MACS/ wiki/Install-macs2 SICER (version 0.1.1) Zang et al. 70 https://github.com/zanglab/SICER2 DiffBind (version 2.10.0) Ross-Innes et al. 71 https://bioconductor.org/packages/ release/bioc/html/DiffBind.html (Continued on next page) Cell Reports 44, 116529, November 25, 2025 23 ..
Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides See Table S3 for the sequence information of qPCR primers, shRNAs, siRNAs and sgRNAs This study N/A Recombinant DNA pMSCV-F-del-Casp9-IRES-GFP Straathof et al. 98 Addgene plasmid# 15567 rtTA-N144 Richner et al. 99 Addgene plasmid# 66810 pXG237 Guo et al. 40 Addgene plasmid# 141281 pRK5-HA-Ubiquitin-WT Lim et al. 100 Addgene plasmid# 17608 pMDLg/pRRE Dull et al. 101 Addgene plasmid# 12251 pRSV-Rev Dull et al. 101 Addgene plasmid# 12253 psPAX2 A gift from Didier Trono Addgene plasmid# 12260 pMD2.G A gift from Didier Trono Addgene plasmid# 12259 pVSV-G Dull et al. 101 Addgene plasmid# 138479 pLKO.1 Moffat et al. 102 Addgene plasmid# 10878 pHAGE-CMV-MCS-PGK-Puro Clontech Laboratories N/A pGEX-6P-1-GST Cytiva Cat# 28954648 pET-28a(+)-HIS MERCK Cat# 69864 pCMV-Myc Takara Bio Cat# 635689 Myc-VCP and mutants This study N/A uORFs-iCasp9-HA This study N/A pHAGE-PGAM5-3xFlag and mutants This study N/A GST-PGAM5 and mutants This study N/A His-OMA1 This study N/A His-PO1 This study N/A HA-OMA1 and mutants This study N/A pHAGE-DELE1-3xFlag This study N/A Software and algorithms Maestro Schrö dinger https://www.schrodinger.com/platform/ products/maestro/ GraphPad Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ GSEA Broad Institute http://software.broadinstitute.org/gsea/ login.jsp ImageJ ImageJ https://imagej.nih.gov/ij/ ZEN 2.3 (blue edition) Carl Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html R version 4.4.0 The R Foundation https://www.r-project.org/ Neuron 114, 1–19.e1–e14, June 17, 2026 e6 ..
Software:Article Title: Nuclear-lamin-guided plastic positioning and folding of the human genome.
Article Snippet: .. This paper N/A Recombinant DNA Cas9 plasmid Addgene Addgene plasmid #87109 pCAG-mCherry-gRNA vector Addgene Addgene plasmid #87110 psPAX2 A gift from Didier Trono Addgene plasmid #12260 pMD2.G A gift from Didier Trono Addgene plasmid #12259 pLgw V5-EcoDam A gift from Bas van Steesel N/A pLgw EcoDam-V5-EMD A gift from Bas van Steesel N/A Software and algorithms Image Lab (version: 4.0) Bio-Rad http://www.bio-rad.com/zh-cn/product/ imagelabsoftware?ID=KRE6P5E8Z ImageJ (version: 1.8.0) NIH https://imagej.net/ij/ FlowJo (version: 10.0.7) FlowJo, LLC https://www.flowjo.com/ Leica LAS AF Lite (version: 2.6.0) Leica N/A GraphPad Prism 9 GraphPad Software Inc. https://www.graphpad.com/ ZEN 3.1 ZEISS https://www.micro-shop.zeiss.com/en/us/ system/zen+software/software+zen/zen++basic+software/410135-1002-310 Phenochart TM 1.0 Phenochart https://www.akoyabio.com/support/ software/phenochart-whole-slide-viewer/ TrimGalore (version 0.6.7) Babraham Bioinformatics https://github.com/FelixKrueger/ TrimGalore R (version 4.0.5 and 3.5) N/A https://www.r-project.org/ pheatmap (version 1.0.12) N/A https://cran.r-project.org/web/packages/ pheatmap/ ggplot2 (version 3.3.5) N/A https://cran.r-project.org/web/packages/ ggplot2/ Metascape (version 3.5) Zhou et al. 62 http://metascape.org/ Fastp (version 0.19.10) Chen et al. 63 https://github.com/OpenGene/fastp featureCounts (version 1.6.4 and 2.0.1) Liao et al. 64 http://subread.sourceforge.net/ featureCounts.html STAR (version 2.7.1a) Dobin et al. 65 https://github.com/alexdobin/STAR DESeq2 (version 1.30.1) Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html ggpubr (version 0.4.0) N/A https://cran.microsoft.com/snapshot/ 2021-09-26/web/packages/ggpubr/index. html deepTools (version 3.5.1) Ramirez et al. 67 https://github.com/deeptools/deepTools Bowtie2 (version 2.2.9) Langmead et al. 68 https://github.com/BenLangmead/bowtie2 MACS2 (version 2.2.6) Zhang et al. 69 https://github.com/macs3-project/MACS/ wiki/Install-macs2 SICER (version 0.1.1) Zang et al. 70 https://github.com/zanglab/SICER2 DiffBind (version 2.10.0) Ross-Innes et al. 71 https://bioconductor.org/packages/ release/bioc/html/DiffBind.html (Continued on next page) Cell Reports 44, 116529, November 25, 2025 23 ..
Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides See Table S3 for the sequence information of qPCR primers, shRNAs, siRNAs and sgRNAs This study N/A Recombinant DNA pMSCV-F-del-Casp9-IRES-GFP Straathof et al. 98 Addgene plasmid# 15567 rtTA-N144 Richner et al. 99 Addgene plasmid# 66810 pXG237 Guo et al. 40 Addgene plasmid# 141281 pRK5-HA-Ubiquitin-WT Lim et al. 100 Addgene plasmid# 17608 pMDLg/pRRE Dull et al. 101 Addgene plasmid# 12251 pRSV-Rev Dull et al. 101 Addgene plasmid# 12253 psPAX2 A gift from Didier Trono Addgene plasmid# 12260 pMD2.G A gift from Didier Trono Addgene plasmid# 12259 pVSV-G Dull et al. 101 Addgene plasmid# 138479 pLKO.1 Moffat et al. 102 Addgene plasmid# 10878 pHAGE-CMV-MCS-PGK-Puro Clontech Laboratories N/A pGEX-6P-1-GST Cytiva Cat# 28954648 pET-28a(+)-HIS MERCK Cat# 69864 pCMV-Myc Takara Bio Cat# 635689 Myc-VCP and mutants This study N/A uORFs-iCasp9-HA This study N/A pHAGE-PGAM5-3xFlag and mutants This study N/A GST-PGAM5 and mutants This study N/A His-OMA1 This study N/A His-PO1 This study N/A HA-OMA1 and mutants This study N/A pHAGE-DELE1-3xFlag This study N/A Software and algorithms Maestro Schrö dinger https://www.schrodinger.com/platform/ products/maestro/ GraphPad Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ GSEA Broad Institute http://software.broadinstitute.org/gsea/ login.jsp ImageJ ImageJ https://imagej.nih.gov/ij/ ZEN 2.3 (blue edition) Carl Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html R version 4.4.0 The R Foundation https://www.r-project.org/ Neuron 114, 1–19.e1–e14, June 17, 2026 e6 ..
Sequencing:Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides See Table S3 for the sequence information of qPCR primers, shRNAs, siRNAs and sgRNAs This study N/A Recombinant DNA pMSCV-F-del-Casp9-IRES-GFP Straathof et al. 98 Addgene plasmid# 15567 rtTA-N144 Richner et al. 99 Addgene plasmid# 66810 pXG237 Guo et al. 40 Addgene plasmid# 141281 pRK5-HA-Ubiquitin-WT Lim et al. 100 Addgene plasmid# 17608 pMDLg/pRRE Dull et al. 101 Addgene plasmid# 12251 pRSV-Rev Dull et al. 101 Addgene plasmid# 12253 psPAX2 A gift from Didier Trono Addgene plasmid# 12260 pMD2.G A gift from Didier Trono Addgene plasmid# 12259 pVSV-G Dull et al. 101 Addgene plasmid# 138479 pLKO.1 Moffat et al. 102 Addgene plasmid# 10878 pHAGE-CMV-MCS-PGK-Puro Clontech Laboratories N/A pGEX-6P-1-GST Cytiva Cat# 28954648 pET-28a(+)-HIS MERCK Cat# 69864 pCMV-Myc Takara Bio Cat# 635689 Myc-VCP and mutants This study N/A uORFs-iCasp9-HA This study N/A pHAGE-PGAM5-3xFlag and mutants This study N/A GST-PGAM5 and mutants This study N/A His-OMA1 This study N/A His-PO1 This study N/A HA-OMA1 and mutants This study N/A pHAGE-DELE1-3xFlag This study N/A Software and algorithms Maestro Schrö dinger https://www.schrodinger.com/platform/ products/maestro/ GraphPad Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ GSEA Broad Institute http://software.broadinstitute.org/gsea/ login.jsp ImageJ ImageJ https://imagej.nih.gov/ij/ ZEN 2.3 (blue edition) Carl Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html R version 4.4.0 The R Foundation https://www.r-project.org/ Neuron 114, 1–19.e1–e14, June 17, 2026 e6 ..
Real-time Polymerase Chain Reaction:Article Title: Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides See Table S3 for the sequence information of qPCR primers, shRNAs, siRNAs and sgRNAs This study N/A Recombinant DNA pMSCV-F-del-Casp9-IRES-GFP Straathof et al. 98 Addgene plasmid# 15567 rtTA-N144 Richner et al. 99 Addgene plasmid# 66810 pXG237 Guo et al. 40 Addgene plasmid# 141281 pRK5-HA-Ubiquitin-WT Lim et al. 100 Addgene plasmid# 17608 pMDLg/pRRE Dull et al. 101 Addgene plasmid# 12251 pRSV-Rev Dull et al. 101 Addgene plasmid# 12253 psPAX2 A gift from Didier Trono Addgene plasmid# 12260 pMD2.G A gift from Didier Trono Addgene plasmid# 12259 pVSV-G Dull et al. 101 Addgene plasmid# 138479 pLKO.1 Moffat et al. 102 Addgene plasmid# 10878 pHAGE-CMV-MCS-PGK-Puro Clontech Laboratories N/A pGEX-6P-1-GST Cytiva Cat# 28954648 pET-28a(+)-HIS MERCK Cat# 69864 pCMV-Myc Takara Bio Cat# 635689 Myc-VCP and mutants This study N/A uORFs-iCasp9-HA This study N/A pHAGE-PGAM5-3xFlag and mutants This study N/A GST-PGAM5 and mutants This study N/A His-OMA1 This study N/A His-PO1 This study N/A HA-OMA1 and mutants This study N/A pHAGE-DELE1-3xFlag This study N/A Software and algorithms Maestro Schrö dinger https://www.schrodinger.com/platform/ products/maestro/ GraphPad Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ GSEA Broad Institute http://software.broadinstitute.org/gsea/ login.jsp ImageJ ImageJ https://imagej.nih.gov/ij/ ZEN 2.3 (blue edition) Carl Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html R version 4.4.0 The R Foundation https://www.r-project.org/ Neuron 114, 1–19.e1–e14, June 17, 2026 e6 ..
|
Addgene inc is a verified supplier 